ESTIMATION 



(I) By distilling with potash, extracting the distilliation with ether, evapo- 

 rating the ether, converting the residue into sulphate and repeating the pro- 

 cess. (2) Tobacco is mixed with aqueous NaOH and some alcohol, and ex- 

 tracted with ether. The extract is evaporated and the nicotine distilled over 

 with steam and estimated by titration with standard acid, or by the polari- 

 meter. 



EXPERIMENTAL. 



OUTLINE OF EXPERIMENTS. 



1. Determination of Total Sulphur and Lime (CaO) in a dilute Lime- 

 sulphur solution of the different strengths as used in the experiments. 



2. Determination of the amount of Nicotine, sulphur trioxide, (SOs ) and 

 the acidity or alkalinity of the different Nicotine preparations used, as Nicotine 

 sulphate, Black Leaf 40, and Nico-fume. 



3. Determination of the soluble Arsenic oxide (As2 O5 ) and the acidity 

 of the arsenate of Lead used. 



4. Mixing of the Lime-sulphur of the different dilutions with the arsenate 

 of Lead at the rate of four pounds of the Lead arsenate to one hundred gal- 

 lons of the solution, and determining the amount of total suphur and Lime 

 (CaO) remaining in solution. Also filtering, drying, and weighing sediment. 

 Using both acid and neutral Lead Arsenate. 



5. Mixing the solutions as in number four and trying the effect of carbon 

 dioxide (CO2 ), as in the carbon dioxide spraying apparatus, on the increase of 

 the soluble Arsenic Oxide (As2 O$ ) and the solution and the decrease of the 

 efficiency of the mixture. 



6. Mixing the solutions of the Lime-sulphur and arsenate lead, again, as 

 in number four and adding 2 cc of the different Nicotine preparations to the 

 mixture and making to a dcfinate volume (1000 cc). Then determining the 

 amount of Total Sulphur and Lime (CaO) remaining in solution. Also filtering, 

 drying, and weighing the sediment. 



7. Effect of carbon -dioxide (CO2 ) as in spraying apparatus upon a dilute 

 Nicotine solution, diluting one to four hundred. 



8. Preparation of Nicotine Sulphate from tobacco stems or poor grade 

 tobacco. 



METHODS OF ANALYSIS 

 DETERMINATION OF NICOTINE 



Solutions required are (a) Alcoholic soda.-Dissolve 6 grams of sodium 

 hydroxide in 40 cc. of water and 50 cc of 90 per cent, alcohol, (b) Sodium 

 hydroxide. -Dissolve 4 grams of sodium hydroxide in 1GOO cc of water, (c) 

 Sulphric Acid. -A standard solution. 



Weigh out from 5 to 6 grams of tobacco extract into a small beaker. Add 

 10 cc of the alcoholic soda solution and follow with enough chemically pure 

 powdered calcium carbonate to form a moist but not a lumpy mass. Mix the 



