228 CLINICAL BACTERIOLOGY AND H^MATOLOGY 



8. Balsam and a cover-glass. 



IV. Staining sections to demonstrate the tubercle bacillus; 

 applicable to the leprosy bacillus also. 



i, 2, 3. Xylol, alcohol, and water, as before. 



4. Carbol fuchsin heated until the steam rises for five 

 minutes or longer, care being taken that the section does not 

 dry up. Or the slide may be immersed in the stain and kept 

 in a warm place for twenty-four hours. 



5. Dilute sulphuric acid until only a faint pink tinge appears 

 after washing. This will generally require an immersion of 

 ten minutes or more. 



6. Methylene blue for three or four minutes. Some of the 

 stain comes out in the alcohol, so that the section must be 

 stained more deeply than will be required ultimately. 



7. Rinse off the blue stain in water, and then remove the 

 greater part of the latter with blotting-paper; this is to 

 render the dehydration more rapid. 



8. Absolute alcohol, two lots in rapid succession. 



9. Xylol. 



10. Balsam and a cover-glass. 



