IMMUNITY 23 



which was dissolved sufficient sodium citrate to make 

 a '5 per cent, solution when the blood was added. 

 This prevented the blood from clotting. By means of 

 a glass funnel, rubber tubing, and trocar and cannula, 

 this citrated blood was slowly injected into the jugular 

 of the diseased cow. The two other cows were treated 

 in a similar manner. Before leaving the premises, I 

 collected from healthy animals 6 quarts of blood. 

 This was allowed to stand in a large glass jar for 

 twenty-four hours, when the serum was decanted off. 

 The amount of serum yielded from 6 quarts of 

 blood varies from 32 to 36 ounces. Twenty-four 

 hours after first bleeding and injection of citrated 

 blood I again bled the animals, this time removing 

 from each 1 quart of blood, and immediately after 

 injecting into each 12 ounces of the serum obtained 

 from the blood of the healthy animals. I repeated 

 injections of normal serum, collected as above, for 

 two more days, when the disease had declined and 

 the animals were well on the way to recovery. The 

 cow treated medicinally died fifty hours after my 

 first seeing her. The three treated by bleeding and 

 administration of normal serum made a complete 

 recovery. 



2. Cow suffering from severe sapremia, due to 

 lacerations of cervix and floor of vagina, which 

 occurred at time of parturition. Temperature on 

 first examination was 105° h\ Adopted treatment 

 same as in No. 1, which was continued for three 

 days, by which time the temperature had fallen to 



