102 YACeiNE- AND SEEUM THERAPY 



and positive results. The scarified area into which 

 the tuberculin had been rubbed usually showed a 

 distinct reaction in tuberculous animals, while animals 

 showed slight inflammatory reddening at the border 

 of the area only in exceptional cases. This test may 

 be applied to any part of the body, by first shaving 

 the hair, but a convenient place for the application of 

 the test is the hairless anal folds at base of tail. 

 Having washed the skin, a 50 per cent, concentrated 

 tuberculin is dropped on the part, and through the 

 drop the skin is scarified with a sharp scalpel. The 

 opposite anal fold acts as a control. In this test 

 previous injections of tuberculin made a short time 

 before may prevent the development of the cutaneous 

 reaction. 



According to collected experiences, the best results 

 are obtained from the subcutaneous method. The 

 intradermal test is next in value to the subcutaneous 

 method. The ophthalmic test is very useful where 

 there is any suspicion of a previous injection being 

 given. This test should be employed in conjunction 

 with the subcutaneous one. 



Specific Paratuberculosis of Cattle (Johne's 

 Disease). — This is a disease of bovines, the seat of 

 the lesions being in the intestines, and characterised 

 by extensive maculate swellings and reddening of 

 the intestinal mucous membrane, clinically charac- 

 terised by chronic diarrhoea and wasting of the 

 animal. The disease is universally distributed, and 

 occurs generally in an enzootic form. 



