104 VACCINE AND SEEUM THERAPY 



The reaction from this method is thermic and local at 

 the site of injection. In diseased animals the tempera- 

 ture is generally at its height from the twelfth to 

 fifteenth hour, but, as with tuberculin, this is not 

 constant, and the mallein reaction may show the 

 same variation as the tuberculin reaction. When the 

 reaction is positive, other symptoms are generally 

 present, such as depression, increased respirations, 

 and loss of appetite, as well as a more or less extensive 

 inflammatory swelling at the point of injection. The 

 mallein reaction is positive if the temperature after 

 injection exceeds the pre-injection temperature by 

 2*7° F. or more and rises above 104° F., provided the 

 pre-injection temperature did not indicate fever. The 

 reaction is positive also if the increase in temperature 

 is only 1-5° to 1*9° F., provided it is attended with 

 a pronounced organic and local reaction ; the local 

 swelling should have a diameter of about 5 inches 

 or more, and the lymphatics in the vicinity of the 

 swelling appear corded and radiate outwards from the 

 periphery of the swelling. The reaction is negative if 

 the rise of temperature does not exceed 1° F. and no 

 organic action occurs. 



2. Intrapalpehral Test. — This was the method used 

 chiefly in the French and English armies throughout 

 the late war in testing horses for glanders. About 

 2 minims of concentrated mallein is injected into the 

 palpebral border of the lower lid of the left eye. A 

 small syringe containing eight doses, with a very fine 

 needle, should be used. Where a large number of 



