122 VACCINE AND SERUM THERAPY 



bacterial emulsion, it can be reduced to the desired 

 number of bacteria in each cubic centimetre. The 

 mixture is then well shaken. The tube is then sus- 

 pended for a period of thirty minutes in a water-bath 

 running at a temperature corresponding with the ther- 

 mal death-point of the bacteria for the vaccine. After 

 removal from the water-bath loopfuls of the emulsion 

 are sown upon suitable media and incubated, in order 

 to determine the sterility of the vaccine. Finally, a 

 small quantity of antiseptic is added to the vaccine, 

 which is then put up for use, either in glass capsules 

 or bottles or tubes closed with a rubber cap. 



Eeferences. 



1. " Serums, Vaccines, and Toxins," Bosanquet and Ej^re. 



2. " Veterinary Bacteriologj'," Buchanan. 



3. " Anaphylaxis and Anti-anaphylaxis," A. Besredka. 



4. " Special Pathology and Therapeutics of the Domesticated 

 Animals, Hutyra and Marek. 



5. The Journal of Immunology. 



6. Veterinary Record. 



7. Veterinary Journal. 



