250 INFECTIONS OF THE DIGESTIVE TRACT 



control animal, and lasted for five hours. Experiments 

 were made which showed that the lengthening effect is 

 not due to a decreased rate of absorption dependent on 

 the depression of the circulation by potassium cyanide. 

 Experiments were also made to determine whether 

 after subcutaneous injections of indol any uncombined 

 indol may be excreted by the gastro-intestinal tract, and 

 if so, under what conditions such excretion occurs. For 

 example, in a dog weighing eleven kilos the small intestine 

 in one foot of its extent (in the region just above the 

 ileocsecal valve) was severed by Dr. Maury from the 

 main gut and the ends sewed into the abdominal wall. 

 The cut ends of the main gut were joined by end-to-end 

 anastomoses. Complete recovery occurred and the 

 health of the dog was apparently perfect. The mucous 

 membrane at the fistulous openings was normal. 

 One month later an experiment was made in which 

 0.5 gm. of indol dissolved in ten cubic centimeters of oil 

 was injected subcutaneously. No symptoms developed. 

 The loop of gut was washed out at intervals of one-half 

 hour during the following nine hours, and no indol could 

 be detected in the distillate by means of the naphthaqui- 

 none reaction. The urine obtained by catheter forty-five 

 minutes after injection of indol gave an extremely intense 

 indican reaction. Repeated examinations showed little 

 falling off in the intensity of the reaction until the lapse 

 of twenty-two hours. After twenty-eight hours the 

 urine showed a normal indican reaction. After an in- 

 terval of two days, in which the animal was apparently 

 normal, he received successive injections of a two per 



