TABLE W 



From this table it will be seen that all of the phosphorus may 

 be determined by this method and that the lecithin will not be 

 broken up. The method used in these determinations is the one 

 adopted for the separation of the inorganic from the organic in the 

 analysis of the dried egg. It is as follows : 



Take 10 grams of the finely powdered substance, extract for 

 three hours with 300 cc. .2 per cent, hydrochloric acid solution. 

 Take an aliquot of this solution after filtering make just basic, 

 then add 15 cc. of magnesium mixture, allow to stand over night, 

 filter through a double filter and wash with 95 per cent, alcohol until 

 free from ammonia, dry the paper and contents, transfer to an 

 Erlenmeyer and digest with 100 cc. of .2 per cent, nitric acid alco- 

 hol solution, filter and take 75 cc. of this soluion, evaporate nearly 

 to dryness, take up with water and a little nitric acid. Filter if 

 necessary. Precipitate with ammonium molybdate and again with 

 the magnesium mixture, finally ignite and weigh as the Mg2P207. 

 This is the method used in obtaining all the results on egg analysis. 



The inorganic phosphorus to be fed to the chickens outside of 

 what is contained in the grain will be in the form of meat scrap or 

 finely ground bone. The following table shows the percentage of 

 phosphorous as inorganic, total, the organic being taken as the dif- 

 ference. The total phosphorus is as P, not the anhydride. In 

 01 der to get a uniform sample of the meat scrap and also the ground 

 bone it was necessary to extract with ether in order to remove the 

 fat so as to grind the sample. The per cent, of phosphorus was de- 

 termined on the original basis. The cabbage as shown in the analy- 

 sis was to be fed as a green food but was withdrawn from the ration. 



The feed given to the one set of chickens will be called organic 

 ration; the other, the inorganic ration. The organic ration com- 

 prises buckwheat 1, wheat 1, pea meal 1-2, cubical grit or ground 



12 



