was added and was shaken at frequent intervals for three 

 tiieri ill ten (!. Suction was required as there was a;i emulsion 

 formed when treated with the acid solutions. To an aliquot por- 

 tion of this just enough ammonium hydroxide was added to make 

 it hasic to litmus paper, magnesium mixture was added without 

 adding the 12 cc. of ammonium hydroxide, Sp. Gr. 90. This was 

 then allowed to stand over night, then filtered and washed with 95 

 per cent, alcohol until free from ammonia. The filter paper and 

 contents were dried, transferred to an Erlenmeyer containing 100 

 cc. of .2 per cent, nitric acid alcohol solution. After standing some 

 time, filter, and take 75 cc. of this filtrate, evaporate nearly to dry- 

 ness, take up with water and a little nitric acid, then precipitate with 

 ammonium molybdate and reprecipitate with the magnesium mix- 

 ture, igniting and finally weighing as the magnesium pyrophosphate. 

 Table Xo. Y will show the complete recovery of the sodium phos- 

 phate by the method just given. 



As the inorganic phosphorus is probably in the form of calcium 

 phosphate a sample of pure calcium phosphate was digested and 

 treated as in the determination of inorganic phosphate. It was 

 found that the .2 per cent, hydrochloric acid would dissolve the cal- 

 cium phosphate and on analysis gave identical results with sample 

 digested with concentrated nitric acid and run by the official method. 



TABLE Y 



The next step was the mixing of Ca3(P04)2, lecithin and starch 

 to determine whether all of the phosphorus could be obtained, if so, 

 there seems to be no doubt that the inorganic phosphate in the egg 

 would be all dissolved by using the .2 per cent, hydrochloric ex- 



t lading solution, as the phosphorus would be in a much finer state 

 of division than the calcium phosphate worked with. Table W 

 will show the results of a series of analvses. 



11 



