matter. Treating this with the neutral ammoniun molybdate in 

 like manner and allowing to stand there was no precipitate formed. 



The egg residue from the extraction with chloroform was treated 

 with the .2 per cent. hydrochloric acid solution on the addition of 

 the ammonium nitrate there was a flocculent precipitate thrown 

 down, this could be cleared up with the addition of strong nitric 

 acid, but on neutralizing this acid and precipitating with the 

 ammonium molybdate there was a small precipitate obtained most 

 of it being the protein bodies thrown down along with a small 

 amount of inorganic phosphorus. 



It seems to be safe to say that the .2 per cent, hydrochloric acid 

 solution when allowed to act on the various portions will not break 

 down the organic phosphorus compounds and yield phosphorus that 

 will be precipitated as the inorganic. 



The next question was to determine whether the magnesium mix- 

 ture with the 12 cc. of ammoninuin hyroxide, Sp. Gr. 0.90, would 

 have any effect upon the hydrochloric acid extract, as this forms 

 with the lecithin an emulsion, a semi-soluble mixture that goes 

 through the filter paper. Taking the lecithin and allowing to 

 stand with the magnesium mixture and 12 cc. of ammonium 

 hydroxide over night there was a ligh flocculent precipitate formed. 

 This did not have the appearance of pure magnesium phosphate. 

 When the residue left after extracting the lecithin is treated in 

 like manner there was no precipitate formed. The residue of the 

 egg after extracting with chloroform was treated similarly and a 

 feint precipitate appeared. On treating them as before but omit- 

 ting the 12 cc. of ammonium hydroxide, Sp. Gr. .90, there was no 

 precipitate 'formed where the lecithin gave one before. The slight 

 precipitate caused by using the strong ammonia in the case of the 

 lecithin is probably caused by saponification of the lecithin, as 

 lecithin yields on saponification fatty acids, phosphoric acid and 

 glycerol, (cholin) 1 . 



In order to find whether or not the .2 per cent, hydrochloric acid 

 solution would separate inorganic phosphorus from a mixture of 

 lecithin and starch. This mixture was used in order to approach 

 the condition of the dry egg. The lecithin was dissolved in ether 

 to get it in a fine state of division so as to be able to get a thor- 

 ough mixture with the starch. The ether was driven off by warm- 

 ing on a water bath. To this mixture was added a known amount 

 of sodium phosphate. The .2 per cent, hydrochloric acid solution 



1. Hammerstein. 



10 



