Four pens containing live 8. c. white leghorn pullets each. 

 All stock to be of uniform age and condition and about six 

 months old al the beginning of the experiment. 



Organic ration : 



The ration employed 1 Ib. buckwheat (whole), 1 Ib. wheat 

 (untreated), 1-2 Ib. pea meal, oyster shell, micro-spar, cubical grit 

 provided. Nutritive ratio 1 :5.6. 



Inorganic ration : 



1 Ib. corn, 1 Ib. oats, 1 Ib. wheat (treated), 1 Ib. ground bone. 

 The bone meal to be fed in a quantity equal to the consumption of 

 oyster shell in first pen. Mierospar cubical grit. Nutritive ratio 

 1:5.4. 



The fowls to be fed the grain rations as much as they will con- 

 sume and no more to be fed until all has been eaten up. The grain 

 is scattered among the litter on the floor. 



The remaining feeds are kept in separate hoppers and weighed 

 at intervals to calculate amount consumed. 



The wheat to be treated with water for twenty-four hours, rinsed 

 and drained one-half hour, then fed : or a quantity treated thus, and 

 dried to prevent fermentation; samples analyzed before and after 

 treatment for organic and inorganic phosphorus. 



The eggs collected" daihvyolks and whites dried and analyzed 

 collectively for organic and inorganic phosphorus. 



The fowls to l>e fed on light diet for several weeks previous to 

 the rations quoted in order to get them as uniform as possible. 



The eggs to be grouped in series of three or four day periods in 

 order to get enough eggs for the analyses and have comparable 

 results. 



The shells collected and analyzed for total phosphorus in similar 

 groups. 



The method of determining the total phosphorus used in the 

 nnalvsis is a modification of the Nonnian method 1 , Concentrated 

 nitric acid was used in the place of ammonium nitrate and a small 

 piece of copper wire was added as it aids in the oxidation and also 

 an indicator when neutralizing with ammonium hydroxide. 



1. DuBois, Reymonds Arcliiv (Physion. Abth.) 1897, p. 552 --V>:}. 



