4O PEACTICAL HISTOLOGY. 



ammonium chromate, and corrosive sublimate. Other methods of 

 preparing the kidney are referred to. (Lesson on Kidney.) 



The bladder is best hardened in chromic acid and spirit mixture, 

 or in Miiller's fluid. 



Harden the spleen, without cutting into it, in Miiller's fluid. 



The suprarenals may be hardened in picro-sulphuric acid. 

 (Lesson on Suprarenal Capsules.) 



Small lymphatic glands from the region of the neck or sul> 

 maxillary region are hardened in alcohol, while others are injected 

 with silver nitrate and osmic acid. (Lesson on Lymphatics.) 



If desired, the large nerve-trunks may be removed and hardened 

 as indicated in Lesson on Nerves, or the smaller branches of nerves 

 may be used for showing the effects of the action of certain reagents 

 on nerve-fibres. 



Remove some of the long bones, leaving in each case the peri- 

 osteum attached to the bone. Cut the bones into pieces about 

 J inch long, and place them for a week in J per cent, chromic acid, 

 and then decalcify them with picric acid, or chromic and nitric 

 acid fluid, or Ebner's fluid. (Lesson XIII.) 



In every case decalcify the ends of the bones, so as to have a 

 section which will demonstrate the relation between the articular 

 cartilage and the osseous tissue. 



Place small pieces of striped muscle in J per cent, chromic acid, 

 and other pieces in alcohol. 



Nerves, with the precautions given in Lesson on Nerves, are 

 hardened in osmic acid, potassic bichromate (2 per cent.), alcohol, 

 or picric acid. 



For the methods of hardening the eye, ear, nose, see the Lessons 

 on these subjects. 



The testis very small pieces is best hardened in Flemming's 

 mixture, and larger pieces in Miiller's fluid. 



For the methods of hardening the ovary, Fallopian tube, and 

 uterus, see the Lessons on these subjects. 



N.B. Label every bottle, and write on the bottle the name of 

 the hardening fluid used, and the dates on which it was changed. 



IX. EMBEDDING. 



This is necessary for many tissues ; the piece of tissue may be 

 either too small to be conveniently held in the hand, or its parts 

 may tend to fall asunder before or after they are cut. 



There are two methods, one simple embedding, where the tissue 

 is simply iixed or placed in another medium to hold it while it is 



