4 8 



PRACTICAL HISTOLOGY. 



Scheme for Infiltration and Embedding (Bohm and Oppel). 

 90 per cent, alcohol. 



Staining in bulk, e.g., borax carmine or hsematoxylin, 

 and washing out the surplus stain. 



Absolute alcohol. 



Toluol or turpentine. 

 Toluol or turpentine-paraffin. 



Paraffin, 

 embedding. 



g 

 I 



5 

 Ether. 



Celloidin solution, No. 3. 

 Celloidin solution, No. 2. 

 Celloidiu solution, No. I. 



Embedding. 

 80 per cent alcohol. 



Object ready to be cut into sections. 



X. SECTION CUTTING. 



1. With a Razor. It is absolutely essential to have a sharp 

 razor if sections are to be cut by hand, and it is well that the 

 student should practise this method. 



Moisten the blade of the razor with 90 per cent, alcohol, and 

 place some alcohol in. an oblong glass vessel. Dip the cutting blade or 

 razor into the alcohol after every third or fourth section. Grasp the 

 razor tightly with the right hand, so that its blade is horizontal, its 

 edge directed to the operator, whose fingers are pressed against the 



