EXAMINATION OF FRESH TISSUES AND FLUIDS. 



93 



tissue elements more distinct. It is used in the form known as 

 Lugol's Solution diluted with water. 



Iodine . 

 Potassic iodide . 

 Water . 



part. 



2 

 100 



Weak-Alkalies (1-3 per cent.) dissolve most tissues with the 

 exception of elastic fibres, pigment, fat, and bacteria. 



The vapour of osmic acid or the fluid itself (i per cent.) may be 

 used. It blackens fatty particles. 



Finally, the tissue may be stained by means of a watery solution 

 of methyl-green, methyl-violet 5B, acetic fuchsin, or methylene-blue 

 in the form of Loffler's Methylene-blue. 



Concentrated alcoholic solution of metliyleiie blue . 30 cc. 

 Caustic potash (o. 10 p. c.). . . . .100,, 



Scheme for Liviny or Fresh Objects. 



Harden the pieces ( IV.). 



Stain in bulk. 



Embed ( IX.). 

 Make sections. 



Fix on slide ( XL ). 



Stain the sections ( XII.). 



Clarify sections ( XIII.). 



Mount sections. 



