138 



PRACTICAL HISTOLOGY. 



[VI. 



Fl0 :.. I f x> ;7: I 11 B P lat 4.? d 



Cihated Cell from the 



7. Isolated Ciliated Epithelium and Goblet-Cells (Frog) (H). 

 Scrape off a little of the epithelium from the mucous membrane of 

 the palate of a frog, which has been macerated 

 for twenty-four hours in dilute alcohol and after- 

 wards stained by picro-carmine. Before staining, 

 it is advisable to place the isolated cells for 

 several hours in | per cent, osmic acid. Diffuse 

 the cells in glycerine., put in a hair, cover, and 

 examine. 



(a.) Observe an isolated ciliated cell ; it is 

 short and columnar, perhaps tapering or divided 

 at one end, while the other end is beset with 

 cilia, resting on a clear, transparent, refractile 

 disc ; the protoplasm is granular, and encloses an 

 oval red-stained nucleus with one or two bright 

 excentrically-placed nucleoli (fig. 99). 



Besides the ciliated cells, there are others 

 without cilia, oval or elongated, pointed at one 

 end. These are from the deeper layers of the 

 ciliated surface (fig. 102). 



(&) Numerous goblet or chalice cells will also 



> ' .. 



of a Frog, be seen, ihey are cup-shaped cells, with an open 

 niouth, and containing mucigen. There is a 

 small amount of protoplasm at one end of the cell 

 the end by which it is fixed which encloses 

 a spherical, oval, or compressed nucleus. The 

 goblet-cells may be seen in two conditions, some clear with their 

 mucigen discharged, and others full of granules or 

 " loaded " with mucigen. 



The cells may also be isolated or dissociated by 

 macerating the membrane in iodised serum (p. 25). 

 A cell isolated in this way, and magnified 1000 

 diameters, is shown in fig. 100. 



8. Isolated Ciliated Cells (Mammal). Use the 

 trachea of a cat or other mammal, and macerate 

 small pieces in dilute alcohol (twenty-four hours). 

 Stain it in bulk in picro-carmine. The isolated cells 

 are examined in glycerine or glycerine-jelly. If the 

 trachea of the ox be used, observe 



(a.) The tall narrow ciliated cells (fig. 101), each 

 with its cilia and clear disc. The ends of the cells 

 may be pointed or branched. Amongst these may 

 be seen oval or battledore-shaped cells (a), the 

 younger cells which exist in the deeper layers of the mucous 

 membrane. 



p. Clear 

 Nucleus ; 

 in. Irregular extre- 

 mity. Iodised serum, 



X 1000. 



. ioi. Ciliated 

 Cells from Tra- 

 chea of Ox. a. 

 Cell from the 

 deeper layers. 

 Dilute alcohol 

 and picro-car- 

 mine, x 300. 



