VIII.] 



CELLULAR AND HYALINE CARTILAGE. 



149 



well-defined membrane bounding the cell-cavity. Not unfrequently 

 several so-called " daughter-cells" may be seen within one capsule. 



6. Hardened Costal Cartilage. Mount a transverse section of 

 costal cartilage which has been hardened in a saturated solution 

 of picric acid. The picric 



acid is removed by washing 

 in alcohol. 



(a.) Stain a section with 

 picro-carmine and mount it 

 in Farrant's solution. 



(/>.) Stain a section in 

 haonatoxylin and mount it 

 in balsam. The matrix is 

 stained of a light blue, and 

 the corpuscles of a deeper 

 tone. 



(c.) A very good stain for 

 hyaline cartilage is MerkePs 

 indigo-carmine stain (p. 67). 



The preparation can be X J 



mounted in balsam, and is 

 not too transparent. 



(d.) Carmine. Place a 

 similar section for twenty- 

 four hours in a strong solu- 

 tion of ammoniacal carmine 

 (p. 63). Wash away the 

 surplus carmine, and allow a 

 drop of strong glacial acetic 

 acid to fall on the section. After a minute or so, wash the section 

 thoroughly in water to remove all the acid, and mount it in Farrant's 

 solution. 



Observe the same arrangement of the cells as before, but the 

 cells are stained red while the matrix is colourless. The connec- 

 tive-tissue corpuscles of the perichondrium are also red. Round 

 each cartilage-cell is a thin outline deeper stained than the rest, 

 indicating the presence of a cartilage-capsule. 



7. Fat in Cartilage-Cells (H). Place a section of costal cartilage 

 (preferably made from a piece of costal cartilage taken from a 

 person over fifty years of uge) in i per cent, osrnic acid for an hour, 

 wash it in water, and mount in Farrant's solution. 



(a.) Observe in some of the cells small and somewhat larger 

 aek spots, which are globules of oil blackened by the osmic 



FIG. ii2. T.S. Human Costal Cartilage, z. Cell 

 shrunk from the wall of its cavity, h ; i. Two 

 cells ia one cartilage-capsule, k; at a; is the 

 commencement of a separation wall ; 2. Five 

 cartilage-cells within one capsule, but the 

 lowest cell has fallen out of its cavity ; 3. Car- 

 tilage-capsule cut obliquely, so that it appears 

 thicker at one side ; 4. Cartilage-capsule not 

 opened into ; g. Hyaline matrix ; /. Fibrous 

 matrix, x 300. 



black 

 acid. 



8. Eosin (H). 



-Stain with a watcrv solution of eosin a section 



