VIII. 



CELLULAR AND HYALINE CARTILAGE- 





capsules. A row of cells may be seen partly in the hyaline and 

 partly in the calcified matrix. Note the finely 

 granular character of the calcified matrix. 



(''.) Observe the bone with its lamellse and 

 bone-corpuscles, and its open meshes containing 

 bone-marrow. 



10. Cartilage of Cuttlefish (H). Mount in 

 Farrant's solution a section of the cephalic car- 

 tilage of a cuttlefish. The cartilage must have 

 been hardened previously in picric acid, alcohol, 

 or osmic acid. 



(a.) Stain a section in picro-carmine. Observe 

 that the cells lie in groups of three or four, and 

 from the periphery of the group processes are 

 given off which anastomose with processes from 

 adjoining groups of cells (fig. 114). 



Eosin and Hsematoxylin. Stain a section 

 slightly with a dilute solution of eosin and after- 

 wards with dilute hsematoxylin. Mount in 

 Farrant's solution. The matrix is reddish, and the cells and their 

 processes purplish in hue. 





FIG. 114. Branched 

 Cartilage-Cells of the 

 Cartilage of Loligo. 

 Picric acid, eosin, aud 

 logwood. 



ADDITIONAL EXERCISE. 



11. Silver Nitrate and Cartilage Matrix (H). Rub a piece of solid silver 

 nitrate upon the cartilaginous end of the freshly-excised femur of a frog. 

 Kxpose the cartilage in water to sunlight. It rapidly becomes brown. Make 

 a thin surface section with a razor, and mount it in Farrant's solution. 



(a.} Observe the matrix stained brown, and a large number of unstained 

 spaces apparently empty. The latter are the cavities in which the cells lie, 

 but the cells themselves are too transparent to be readily seen. This picture 

 is the reverse of that obtained with gold chloride (p. 147). 



LESSON IX. 

 THE FIBRO-CARTILAGES (WHITE AND YELLOW). 



III. Fibro-Cartilages. 

 A. White Fibro-Cartilage. 



1. Intervertebral Disc. Decalcify in chromic and nitric acid 

 fluid (p. 37) or picro-sulphuric arid (24 hours) an intervertehral disc 



