154 



PRACTICAL HISTOLOGY. 



[IX. 



tissue, with numerous elastic fibres ; the latter can be traced into, 

 and become continuous with, the elastic fibres of the matrix (iig. 

 117, e, /). The matrix consists of fine branched and anastomosing 

 fibres of elastic tissue, stained yellow with picric acid. Where the 

 fibres are cut transversely, they appear as yellow dots or granules. 



In addition to these, however, there 

 are numerous granules of elastin 

 scattered in the matrix. In this 

 meshwork notice the nucleated 

 cells stained red. Each cell has a 

 capsule, but near the perichondrium 

 they are smaller and flattened (/), 

 while in the substance of the carti- 

 lage they are larger, oval, or 

 spherical (?). 



5. Acid Fuchsin Method. Stain 

 a section with a watery solution of 

 acid fuchsin. Wash the section for 

 a long time in absolute alcohol and 

 mount in balsam. The network is 

 intensely red, and the other parts 

 uncoloured. 



6. Double Staining of the Epi- 

 glottis. (i.) Stain a section Avith 

 picro-carmine, and then faintly with 

 logwood. Mount in balsam. To 

 preserve the yellow colour of the 

 fibrils, the clove-oil, with which the 

 section is cleared up, must be made 

 yellowish by dissolving in it a little 

 picric acid. The fibres are yellow, 

 the cells red, and the nuclei 

 purplish. 



(ii.) Stain another section with 



,.- ,~ >, .- , ,, ~ dilute eosin-hsematoxylin, and mount 



Elastic fibres ; i. Superficial layers of it in Farrant's solution or balsam. 



The cells take the logwood tint, and 

 Alcohol and picrb- the fibres the colour of eosin. 



7. Ear of Pig or Horse. Stain 

 a section in picro-carmine, and mount it in Farrant's solution. 



(L and H) Observe the skin, its glands and muscles. Neglect 

 these, and note the perichondrium enclosing the cartilage with a 

 characteristic arrangement of the cells. The cells near the surface 

 are small, flattened, and parallel to it, while those in the centre 

 are larger and arranged across the long axis of the section. 





n 



FIG. 117 T S. Epiglottis 



Fat-cells in perichoudrimn 



f a Dog. 

 c 



smaller cells ; r. Layer of larger 

 cells with elastic grannies, l.\ f. 

 Pericliondrium. 

 carnune. 



