164 



PRACTICAL HISTOLOGY. 



[XI. 



(b.) The branching stellate spaces, interfascicular spaces (fig. 

 129, c), between the fasciculi or bundles of fibres. If these spaces 

 contain air, they appear somewhat dark. These spaces can readily 

 be seen as branched dark spaces if a transverse section is made 

 by means of a knife, not a razor of a small tendon dried at the 

 ordinary temperature. 



(c.) (H) Observe the cut ends of the fibres (I), which appear 

 almost homogeneous, but amongst them here and there may be 

 seen a few dots, which are the transverse sections of elastic fibres. 



and the branched tendon- 

 spaces (c), some of them 

 with a nucleated branched 

 cell. 



2. L.S. Tendon. Stain 

 a section with logwood, and 

 mount it in balsam. (H) 

 Observe the longitudinal 

 arrangement of the fibres, 

 and between them rows of 

 fusiform tendon - cells, or 

 rather the long fusiform 

 nuclei of the tendon cells 

 arranged between the 

 fibres. The other parts of 

 the cell become too trans- 

 parent to be seen (fig. 130). 

 Sometimes a L.S. of one of 

 the septa may be seen. 



3. Fibrils in Tendon 

 (H). Macerate a tendon 

 from the tail of a rat for 

 twenty-four hours in a satu- 

 rated solution of picric acid, 

 or for 34 hours in baryta 

 water. Tease a small part, 

 and examine it first in 



water. Mount it in Farrant's solution. This is apt, however, to 

 render the fibrils too transparent. Perhaps a better method is to 

 place the fine tendons for twenty-four hours in equal parts of 

 i per cent, osmic acid and i per cent, silver nitrate. Mount a 

 teased preparation in Farrant's solution. 



(.) Observe the isolated fibrils (-^-Q^-Q-Q inch in diameter), exces- 

 sively fine; wavy, and unbranched (fig. 131). 



4. Tendon of Eat (Gold Chloride Method). Kill a rat, cut off 

 its tail, forcibly rupture the tail, when a long leash of fine white 



FIG 



u 



Rows of nuclei of tendon-cells. 



