1 66 



PRACTICAL HISTOLOGY. 



[XL 



(b.) If a side view of the cells is obtained (fig. 132, a), they 

 partially clasp the fibre, but never envelope it completely ; in this 

 respect these cells differ from endothelium. 



5. T.S. of Gold Tendon (H). Kemove the skin from the tail of 

 a young rat, cut out a piece of the tail a quarter of an inch in 

 length with its tendons, and subject it to the gold chloride process 

 (p. 79). When, after reduction, it has become purple or brownish, 

 decalcify the bone, harden it in alcohol, and make transverse 

 sections. Mount one in balsam. 



(a.) Many tissues will be seen, including muscle, nerve, fat, and 

 bone. Neglecting these, observe the small rounded areas at the 

 circumference, the transverse sections of the small tendons, each 

 surrounded by its own sheath of connective tissue (fig. 134, t). In 

 each observe the branched stellate spaces (fig. 134, c), frequently 

 anastomosing with each other. These interfascicular spaces are 



..I 



FIG. 134. T.S. of a small Ten- 

 don, Tail of Kat. t. Sheath ; 

 c. Interfascicular spaces 

 with tendon -celU. Gold 

 chloride. 



thellal Cells on the Sur- 

 face of a Tendon, Tail of 

 Rat. Silver nitrate. 



purplish in colour ; they contain the tendon-cells, and also a 

 purplish deposit due to the gold chloride acting on the lymph which 

 they contain in the fresh condition. 



6. Endothelial Sheath of Tendon (L and H) Silver a leash of 

 the fine tendons from the tail of a rat. Mount a short length of 

 one of them in balsam. 



(a.). Observe the tendon made up of parallel fibres, and note on 

 their surface a single layer of endothelium. The squamesare large, 

 polygonal, and mapped out by "silver lines," but no nuclei are 

 visible. 



7. Fresh Tendons and Acid Logwood (H).- Place three or four 

 tendons (rat's) i| inch long, on a dry slide, and fix their ends with 

 paraffin, so as to keep them extended. Make a solution of acid 

 logwood by adding one part of i per cent, glacial acetic acid to 

 three parts of. logwood solution. This solution is red. Place a 

 drop of it on a cover-glass, and lay it on the tendons. The acid 

 brings into view rows of narrow, granular, nucleated cells between 



