XV.] 



MUSCULAR TISSUE. 



lyi 



connective tissue. The fasciculi are large or small according to the 

 number of fibres entering into their composition. 



(b.) In some preparations one can see intercellular bridges between 

 adjacent cells, like those that occur in squamous epithelium. 



4. L.S. of Non-Striped Muscle (H). This may be obtained by 

 making a longitudinal section of 

 the longitudinal coat of the in- 

 testine, but it is better to strip 

 off a thin lamella of this coat from 

 the intestine of a rabbit hardem-d 

 in spirit or Muller's fluid. Stain 

 in hsematoxylin and mount in 

 balsam. 



(.) Observe the oval fusiform 

 nuclei (fig. 164, n) lying in narrow 

 fusiform areas the cells. The 

 boundary-lines between the cells 



FIG. 



164. 

 estim 



A. T.S. non-striped muscle, in- 

 testine of cat ; B. Longitudinal strip of 

 intestine of rabbit; c. Cell; n. Nucleus; 

 s. Septum of connective tissue. Chro- 

 mic and b chromate fluid, hsematoxylin. 



are usually not well defined. 



5. Cement Substance of Non- 

 Striped Muscle (H). With dis- 

 tilled water wash out the contents 

 of the small intestine of a freshly-killed rabbit, or the large intes- 

 tine of a frog. Tie one end of the gut, and fill it with .5 per cent, 

 solution of silver nitrate, and tic the other end of the gut. Suspend 

 the whole in J per cent, solution of silver nitrate for ten minutes 

 or so. Slit up the gut along the line of 

 attachment of the mesentery. Wash it 

 in water and expose it to light. It soon 

 becomes brown. Lay it on a glass plate, 

 mucous surface uppermost, and with a 

 scalpel* scrape away all the mucous and 

 submucous coat, which is very easily 

 done, especially if the intestine has been 

 macerated for about twenty-four hours in 

 water. There remain only the muscular 

 and thin serous coats. Harden in alco- 

 hol. Snip out a piece, dehydrate com- 

 pletely in absolute alcohol, and mount in FIG. 165. Cement Substance of 



L i Smooth Muscle, Intestine of 



Rabbit. Silver nitrate. 



(a.) Observe the narrow elongated 



fusiform areas bounded by silver lines (fig. 165) ; they indicate the 

 outline of the fusiform cells. On focussing upwards and down- 

 wards, notice the longitudinal and circular direction of the fibres 

 crossing each other. In this preparation also many lymphatic 

 paths lined by sinuous epithelium may be seen. They are recog- 



