1 S 8 



PRACTICAL HISTOLOGY. 



[XVI. 



8. T.S. of Muscle. Make transverse sections of a small mamma- 

 lian muscle which lias been kept stretched and hardened in 0.5 per 

 cent, chromic acid and afterwards in alcohol. Stain one in logwood 

 and mount it in Canada balsam. 



(a.) (L) Observe the sheath of connective tissue or perimysium 

 surrounding the whole muscle, and that from it septa pass between 

 groups or fasciculi of the muscle-fibres, and also a small amount 

 between the muscle-fibres, forming the endomysium (figs. 173, 

 174). 



The ends of the muscular fibres somewhat polygonal or rounded, 

 with stained nuclei (one, two, or three) immediately under the 

 sarcolemma. In amphibian muscles (fig. 174, A) and in a few 



mammalian muscles, e.#., 

 the semi-tendinosus of 

 the rabbit, nuclei also 

 occur within the sarcous 

 substance. 



(6.) (H) The ends of 

 the fibres appear finely 

 dotted with clear inter- 

 spaces, the dots corre- 

 sponding with the ends 

 of the bundles of fibrils 

 or muscle - prisms or 

 sarcostyles, while the 

 clear areas are due to 

 what is called sarcoglia. 

 9. L. S. Injected 

 Muscle (L). Make a 

 longitudinal section of 

 an injected muscle, i.e., 

 parallel to the direction 

 of its fibres. It is better 

 to inject the whole of 

 the posterior half of the 

 body, e.g.) of a rabbit, 

 from the aorta. The 

 injected bone will yield injected muscle. 



FIG. 176. T.S. and L.S. Injected Striped Muscle. 

 and 2. T.S. Muscular fibres ; 3. L.S.; a. Arteries. 



preparation made for 

 Mount it in balsam. 



(a.) Observe the elongated quadrilateral meshes of capillaries 

 between the muscular fibres, but outside the sarcolemma, and that 

 capillaries run between the fibres with short transverse connecting 

 branches. Trace their origin from an artery and their termination 

 in a vein (fig. i 75). 



10. T.S. Injected Muscle. Mount, either strained or unstrained, 



