XVIII.] 



NERVE-GANGLIA, NERVE-CELLS, ETC. 



221 



cylinder, one sees its branched arborescent terminations in the protoplasm 

 (tig. 205). A good plan to see the unaltered end-plates is to examine the 



Nerve-fibre. 



End-plate. 



Muscle nucleus. 



FIG. 204. Termination of a Xerve-Fibre in End-Plate of a Lizard's Muscle. 



muscle in a freshly-prepared I per cent, solution of sulphate of iron or ammonio- 

 sulphate of iron (Kiihnc, Mays}. 



14. Frog's Motor Nerve-Endings. There is no " end-plate," as in mamma- 

 lian muscles, but the axis-cylinder splits up into bayonet-shaped branches 

 under the sarcolemma. The myelin is continued up 



nearly to the sarcolemma, but as the nerve-fibre 

 perforates the sarcolemma and becomes hypolemmal, 

 the myelin stops, so that within the sarcolemma the 

 branches are pale, and consist of branches of the axial- 

 cylinder only. The gold method (p. 79) may be used. 

 A simple method is to stain a small piece of a fresh 

 muscle (e.g., sartorius, near its middle, not at the ends) 

 with Delafield's logwood. It stains the hypo-sarco- 

 lemmal nerve-terminations. The mode of terminal ion 

 of nerves in sensory surfaces will be found under Skin, 

 Eye, and the sense-organs generally. 



15. Terminations of Nerves in Tendon. This is 

 best shown by one of the gold- chloride methods. 

 Perhaps the following by Manfredi is the best : 



Place the tendinous ends of the gemelli muscles of a 

 rabbit (or the enucleated eyeball with its muscles 

 attached) in 



Potass bichromate (2 per cent.) . for 3 days. 



Acetic or arsenic acid (i per cent.) . ,, 30 mins. 



Gold chloride ( i per cent.) ... ,, 30 ,, 

 Wash and leave exposed in sunlight in I per cent, arsenic acid until it assumes 

 a violet-blue colour. 



16. Pyriform Nerve-Cells (Frog). These are most readily found in the 

 ganglion of the vagus as it issues from the skull. Pith a frog, distend the 

 (esophagus by pushing a small test-tube into it, place the frog on its belly, 

 relleet the skin over the shoulder-blade, divide the trapezius and remove the 

 fore-limb. The vagus will be seen coming out, along with the glosso-pharyn- 

 geal, through a large foramen immediately in front of the occipital condyle. 

 Clear away the muscles from the region of this foramen, snip and excise a 



Fro. 205. End-Plate of 

 a Lizard's Muscle. 

 One end -plate seen 

 in profile, the other 

 from above, with a 

 nerve - fibre axis - 

 cylinder terminating 

 in it. Gold chloride, 

 Golgi's method. 



