262 



PRACTICAL HISTOLOGY. 



[XXIII. 



j. 253. Human Sub-Maxillary Gland. On the right 

 groups of mucous, and on the left of serous 

 alveoli, x 300 



C. Muco-Salivary Glands, e.g., sub-maxillary of man or retro- 

 lingual of the dog, are treated as the other salivary glands. 



6. Human Sub-Maxillary Gland. (L and H) Observe'that some 

 of the acini are like those of mucous glands, and others like serous 

 acini, while some of the acini contain both mucous and serous 



cells. Acini, serous and 

 mucous, may be found 

 lying side by side (fig. 



253). 

 7. Dog's Sub-Maxillary 



Gland (Double-Staining). 



(i.) Stain alcohol-hard- 

 ened sections, first in a 

 watery solution of aniline 

 green and subsequently in 

 eosin. An easier plan is 

 to stain first in aniline 

 green, ' rapidly dehydrate 

 the section in alcohol, 

 taking care that all the 

 green is not washed out 

 of the gland-cells, and clarify with clove-oil in which is dissolved 

 some eosin. Mount in balsam. The mucous cells are green, the 

 demilunes pinkish, and the nuclei generally are green. The cells 

 of the ducts are green, and the interlobular connective tissue 

 pinkish, 



(ii ) Stain sections in aniline blue, to which is added a saturated 

 watery solution of picric acid. Mount in balsam. The cells of the 

 acini are blue, while the ducts are yellowish -green. 



(iii.) Schiefferdecker's method is also to be recommended. Add 

 a few drops of a 5 per cent, alkaline alcoholic solution of eosin to 

 a watch-glassful of alcohol. Allow the sections to stain in this for 

 half an hour or so, and place them for a few minutes in i per cent, 

 watery solution of aniline green, and mount in balsam. 



(iv.) The sub-maxillary gland of a dog or guinea-pig hardened in 

 picric acid or HgCl 2 , if stained with aniline blue and safranin, 

 shows the demilunes red and the mucous cells blue in balsam 

 preparations. 



THE PANCREAS. 



The pancreas is a compound tubular gland, and resembles the 

 serous salivary glands in the arrangement of its capsule, lobes, 

 lobules, and duct, with its branches. The epithelium of its ducts, 

 however, is not so distinctly striated, and in a section, as a rule, 



