266 PRACTICAL HISTOLOGY. [XXIV, 



fluid, stain with safranin and wash with alcohol containing picric acid. The 

 safranin stains the granules. 



(b.) A pancreas fixed in Flemming's fluid or sublimate is beautifully stained 

 by means of the following modification by Oppel of Bioudi's fluid : 



Meth yl -green ( I per cent.) . . . 120 cc. 

 Eosin(i percent.) .... 2 ,, 



Acid-fuchsin (i per cent.) . . . 40 ,, 

 Absolute alcohol 3 . . . 40 ,, 



The granules are red and the nuclei green. 



22. Terminations of Nerves in Pancreas. By means of Golgi's rapid 

 hardening method (Lesson XXX.) i.e., osmico-bichromate fluid and then 

 silver nitrate Ramon y Cayal and Sala, 1 and more recently Erik Miiller, 2 

 have traced the terminations of nerve-fibres in the pancreas (dog, rabbit). 

 Numerous nerve-fibres enter the pancreas along its ducts, and terminate in a 

 rich plexus of fibrils around the individual acini. The nerve-fibres which 

 surround the acini are derived from two sources, some fibre fibrils are branches 

 of Remak's fibres, and others are processes of very characteristic cells, which 

 R. y Cayal calls "visceral sympathetic ganglion cells." These cells send off 

 processes which are arranged as part of the "peri-acinous" plexus, and some 

 of them penetrate between the gland-cells. Miiller finds that the fibrils lie in 

 relation with the secretory cells. 



LESSON XXIV. 

 THE STOMACH. 



THE walls of the stomach, like the intestine, are composed of four 

 coats, named from within outwards 



(i.) Mucous coat, i.e., the glandular coat. 



(2.) Submucous, composed of loosely-arranged connective 



tissue, the larger blood-vessels, lymphatics, and nerves. 

 (3.) Muscular, composed of three layers of non-striped muscle 



(a.) longitudinal, (b.) circular, (c.) oblique. In some 



situations only two layers exist. 

 (4.) Serous, from the peritoneum. 



The mucous coat is lined by a single layer of tall, narrow, cylin- 

 drical mucous cells in reality mucus-secreting goblet-cells for 

 they have open mouths and contain mucigen. The cardiac portion 

 of the mucous membrane is composed of tubular glands fundus 

 glands placed side by side. Several gland-tubes may open into 



1 Termination de los nervios y tubos glandulares del pancreas de los verte- 

 Irados, Barcelona, 1891. 



a Archivf. mik. Anat., xl. p. 405, 1892. 



