274 



PKACTICAL HISTOLOGY. 



[XXV. 



cells, the nucleus near the attached end of the cell. There is 

 a gradual transition from these cells to those covering the villi. 

 A clear border may be seen on their free ends. Bizzozero has 

 called these "protoplasm cells" to dis- 

 tinguish them from the goblet-cells which 

 lie amongst them. Goblet-cells, however, 

 are far more abundant in the large intestine. 

 The lumen of each gland is distinct, and, 

 especially if the lining cells be raised 

 slightly, the basement membrane of the 

 gland-tube may be seen. Between the 

 gland-tubes numerous leucocytes and 

 adenoid tissue ; in fact, the glands are set 

 in a mesh work of this tissue. 



(i.) The muscnlaris mucosse sends deli- 

 cate processes into the villi. This is best 

 recognised in balsam specimens, by the 

 arrangement of the fusiform nuclei of the 

 smooth muscle cells. 



(/.) A solitary gland or a Peyer's patch 

 may be cut, but it is better to have special 

 preparations for these. 



2. Peyer's Patches or Agminated 

 Lymph Follicles. (i.) Make V.S. through 

 a hardened Peyer's patch (sublimate or 

 alcohol). Stain with eosin and hsemato- 

 xylin and mount in balsam. 



(ii.) Fix a Peyer's patch of a rabbit or 

 guinea-pig in Flemming's fluid. Fix a sec- 

 tion on a slide and stain it first in i per cent, 

 aniline-blue (watery). Wash out fn i per 

 cent, ammonia, then in .5 per cent. HC1, 

 and stain in safranin (Garbini). 

 in balsam. 



(L) Observe a group of oval or roundish 

 masses of adenoid tissue crowded with 



Mount 



FIG. 260. T.S. Small Intestine 

 (Cat). V. Villi ; LG. Lieber- 

 kiilm's glands; MM. Mus- 

 cularis mucosse ; C and L. 

 Circular and longitudinal 



fibres of muscular coat; s. leucocytes confined to one side of the gut. 

 The conical apices of some of them may be 



seen projecting upwards quite to the mucous surface, covered only 

 by a layer of columnar epithelium. Between the epithelial cells 

 may be seen colourless corpuscles which have wandered from the 

 adenoid mass. The lower ends of the masses usually pass down 

 into the submucous coat. No villi exist over the apices of these 

 masses of adenoid tissue if they project well into the mucous layer. 

 If, however, they do not, but exist merely as rounded masses of 



