350 



PRACTICAL HISTOLOGY. 



[XXXI. 



cut at right angles to the direction of the folds. Stain it with 

 carmine, aniline blue-black, or, better still, first with logwood and 

 then with eosin. Mount in balsam. The best way for the student 

 is to stain a small piece of the cerebellum in bulk in borax- 

 carmine for two or three days, or longer, and then embed and 

 cut in paraffin. In this way there is no fear of the sections break- 

 ing up, and the relative positions of the several parts are accurately 

 maintained. 



In making sections of the cerebellum, it is important, if one 

 wishes to see the wide expanse of the protoplasmic processes of 

 Purkinje's cells, to make sections across the direction of the laminae. 

 If made in the direction of the laminae the leash of protoplasmic 

 processes appear quite narrow (fig. 330, B). The protoplasmic 

 processes, or dendrites, as they have been called by His, spread out 

 in planes transverse to the direction of the lamella?, hence the 

 necessity for the above precaution. 



Suppose a hsematoxylin-eosin specimen to be prepared. 



(a.) (L) Observe the primary and secondary convolutions (fig. 

 325). In each leaflet from within outwards, the white matter, 



FIG. 325. Leaflet of the 

 Human Cerebellum, 

 x 10. 



FIG. 326. Cortex of the Cerebellum, x 90. 

 . Outer; b. Inner or granular layer: 

 p. Cells of Purkinje. 



composed of medullated nerve-fibres, and outside this the grey 

 matter, composed of two layers, viz. : (i.) The nuclear layer, 

 composed of many layers of small nuclei stained blue eacli 

 surrounded by a very small quantity of protoplasm. (ii.) The 

 outer layer of the cortex, thicker than (i.), with a somewhat 

 granular appearance, and containing branches of Purkinje's cells 

 and some small branched angular nucleated cells (fig. 326). 



