XXXIIL] 



THE NOSE. 



375 



(24 hours). Harden in alcohol, make V.S., stain with haematoxylin, 

 and mount in balsam. 



(a.) (L) Observe on the surface the row of columnar epithelial 

 cells with oval nuclei, and under these numerous rows of cells with 

 spherical nuclei. At the base of the latter a row of more granular- 

 looking basal cells (fig. 344). The basis of the mucous membrane 

 composed of connective tissue with sections of numerous glands 

 Bowman's Glands. Sections of blood-vessels and branches of the 

 non-medullated olfactory nerve may also be seen. 



6. Isolated Olfactory Cells. Place small pieces of the olfactory 



FIG. 344. V.S. Olfactory Region (Rabbit), s. Disc 

 on cells ; zo and zr. Zones of oval and round 

 nuclei ; b. Basal cells ; dr. Part of Bowman's 

 glands ; n. Branch of olfactory nerve. 



.FIG. 345. Olfactory 

 Cells. N. Ilumau; 

 n. Frog ; E. Sup 

 porting cell. 



mucous- membrane in dilute alcohol (24 hours). Fix the membrane 

 in i per cent, osmic acid (5 minutes). Stain the pieces in bulk in 

 picro-carmine (24 hours). Scrape off a little of the stained and 

 softened epithelial covering and mount it in glycerine. 



(H) (a.) Observe the olfactory cells as very narrow, cylindrical, 

 elongated cells with a large spherical nucleus, much broader than 

 the body of the cell. The free surface carries fine cilia, but they 

 are apt to be displaced in the process of teasing the tissue (fig. 345). 



(/>.) Supporting cells, not unlike columnar epithelium, but they 

 have a large oval nucleus (fig. 345, E). They have a long central 



