388 PRACTICAL HISTOLOGY. [xxxvi. 



(a.) Observe numerous sections of the twisted tube of the 

 epididymis (fig. 359); each tubule has a fibrous wall, and is lined 

 by a single layer of tall, slender, columnar ciliated epithelial cells. 

 Each cell is provided with a fringe of long cilia. The lumen is 

 wide and distinct} and may contain a confused mass of spermatozoa. 

 At the bases of the cells are sometimes seen small cells, and in 

 pigmented animals pigment granules are not unfrequently seen in 

 the connective-tissue stroma supporting the tubule. There is a 

 fair amount of connective-tissue stroma between the tubules, and 

 in it are blood-vessels, lymphatics, and nerves. 



10. T.S. Vas Deferens. Harden it like the epididymis, make 

 T.S., stain, and observe the arrangement of its parts as described 

 at p. 383. 



LESSON XXXYI. 

 THE OVARY FALLOPIAN TUBE UTERUS. 



THE OVARY. 



THE ovary consists of a stroma, covered on the surface with a 

 single layer of short columnar or germinal epithelium. Embedded 

 in the stroma are numerous Graafian follicles in all stages of ripe- 

 ness. Each Graafian follicle contains one ovum. The unripe 

 follicles, with primitive ova, form a superficial layer close under 

 the surface, while the more mature ova and follicles lie deeper. 

 The coverings of the follicle and the structure of a ripe ovum are 

 given at p. 389. 



Methods. Select the ovaries of small animals, e.g.) mouse, rat, 

 as they can be better "fixed" than large ones. 



(i.) Harden the ovary of small animals in toto in Mtiller's fluid 

 (2 weeks), and then in alcohol. Make two or three transverse 

 cuts in the human ovary before hardening it in the same fluid. 



(ii.) Picro-sulphuric acid, for a day, is also a good hardening 

 reagent. Complete the hardening in alcohol. The pieces had 

 better be stained in bulk in borax-carmine, or Kleinenberg's 

 logwood, and embedded and cut in paraffin, or embedded and cut 

 in celloidin. 



(iii.) For small ovaries use Flemming's fluid, embed and cut in 

 paraffin. Stain with safranin. 



For obtaining a general view, the sections must be pretty thick, 



