360 



UNIVERSITY OF CALIFORNIA EXPERIMENT STATION. 



Experiment II. Made further test of resistance to desiccation on 

 December 18, 1908, under similar conditions to those indicated under 

 Experiment I. The following table shows tabulated results : 



The experiment showed very few tubes with the blight organism alive 

 even after three days, but some of the tubes show growth of the organism 

 even after twenty days. 



Experiment 777. January 2, 1911. Used a three-day-old culture 

 growing in a meat peptone glucose bouillon made as follows : 5 gm. 

 Liebig meat extract, 5 gm. NaCl, 10 gm. Witte's peptone, 1,000 c.c. 

 distilled water, 5 gm. glucose (Merck). Tfiis same medium was also 

 used in which to test the desiccated cover glasses. A loopful of culture 

 was placed on each sterilized one half inch cover glass, using a 2 mm. 

 loop on the platinum needle. These covers were dropped into the above 

 medium at given intervals of time, as is shown in the following table : 



The inoculated tubes were incubated at 20 to 25 C., as well as the 

 inoculated potato cylinders. The desiccating cover glasses were kept at 

 about 15 C. This organism belongs to a genus in which are several 

 yellow chromogenic organisms that are quite resistant to drying. 



Experiment IV. This experiment was a combination of desiccation 

 and influence of light. March 9, 1908, a tube of Dunham solution was 

 inoculated. After three days' growth at 20 C. this liquid was atomized 

 on living olive shoots about one year old. 



Series 1. The same liquid was atomized on a small English walnut 



