23 



0.3895 gram of the substance obtained by peptic digestion 

 of osseoalbumoid (A, 2, b) was dissolved in 6 cc. of 0.85 per 

 cent. NaCl, which were then injected into the loose cellular 

 tissue of the right flank at 11.50 A.M., Nov. 27, 1908. 



Weight of the dog: 9.65 kilos; temperature of the dog: 

 101.20. Amount of substance injected per kilo of dog: 0.040 

 gram. 



The dog showed great lassitude for about seven hours after 

 the injection. 1 



Certain urinary data are given in Table I on page 24. 



The remainder of each fraction of urine after the following 

 tests had been made was saturated with (NH 4 ) 2 SO 4 at room tem- 

 perature and the precipitate collected. Fractions No. 2 and 

 No. 3 gave small amounts of proteose-like precipitates, and 

 fraction No. i a still smaller yield. These precipitates were 

 dissolved in water, filtered and the filtrates tested with the 

 results shown in Table II (page 24). 



The filtrates from the precipitates obtained by saturation 

 with (NH 4 ) 2 SO 4 at room temperature (Table II) were further 

 saturated with (NH 4 ) 2 SO 4 at the boiling temperature. The 

 precipitates were collected, dissolved in water, filtered and 

 tested with the results shown in Table III (page 25). 



1 The urine was collected before the injection (after the purulent 

 material from the eyes had been ruled out as a factor in the reaction with 

 potassio-mercuric iodide) and preserved with thymol. As it was sub- 

 sequently thought that perhaps this preservative might account for 

 the reaction obtained, urine that did not give the test was preserved 

 with thymol, and allowed to stand for twelve hours. It then gave a pre- 

 cipitate with potassio-mercuric iodide, which dissolved on further heating 

 and reappeared on cooling. The same urine gave no precipitate with 

 picric acid or trichloracetic acid, however, This test was repeated sev- 

 eral times with the same result. 



(The nature of this reaction is being studied and will be the subject 

 of a future communication.) 



