26 



Second Experiment. The subject of the preceding experiment 

 received another subcutaneous injection of 1.1460 gram of 

 osseoalbumoid (A, 2, c). The amount of injected sub- 

 stance per kilo of the dog's body-weight was 0.1187 gram. 

 The dose was dissolved in 8 cc. of 0.85 per cent. NaCl. This 

 was injected at 12.05 P.M., Nov. 28, 1908. The dog's tem- 

 perature was 101.3 F. No untoward symptoms developed. 

 Certain urinary data are given in Table IV (page 25). 



The fractions of urine from this dog, which are referred 

 to in Table IV, were saturated with (NH 4 ) 2 SO 4 at room tem- 

 perature and a considerable amount of proteose-like material 

 was collected from each. This was filtered off and each 

 filtrate saturated with (NH 4 ) 2 SO 4 at the boiling temperature, 

 yielding a very small amount of precipitate. The precipitates 

 were dissolved in water, filtered and tested as follows : 



Fraction. (NH^jSO* (cold). (NH 4 )aSO 4 to filtrate (boiling). 



No. i Considerable amount Very small amount of 



of proteose-like pre- precipitate, 

 cipitate. 



No. 2 



Nos 3, 4 No proteose-like ma- No precipitate, 

 terial ; very small 

 amount of dark gran- 

 ular precipitate. 



Nos. 5, 6 



Solutions of these precipitates of fractions i and 2 were 

 dialyzed free from sulphate and poured into alcohol. A con- 

 siderable amount of white, flaky precipitate came down. 

 This precipitate, after standing under alcohol for two months, 

 was found to have become quite insoluble. Sulphate was 

 present, for the water with which the precipitate was washed 

 gave a good reaction with BaCl 2 . The washings gave negative 

 results with the Millon and the xanthoproteic tests but the 

 insoluble precipitate gave reactions with both. The washings, 

 however, gave a biuret test, which may have been due to 

 the presence of urobilin (Salkowski, Berl. Klin. Woch. t 1897, No. 

 J 7 P- 353)j or to a small amount of the precipitate that was 

 soluble in the water. No precipitate appeared on heating 

 the washings from the insoluble precipitate. 



