28 



The results of the above experiment indicate that the 

 (NH 4 ) 2 SO 4 precipitate from the urine contained the substance in- 

 jected into the dog and that this substance was rendered in- 

 soluble when precipitated by means of alcohol from its aqueous 

 solution. One of the reasons for supposing that the Bence Jones 

 protein more nearly resembles the "primary proteins" than the 

 " secondary protein derivatives," is the fact that the albumins 

 and globulins are rendered insoluble on standing under alco- 

 hol while the proteoses and peptones are not.. The result 

 referred to above seems to indicate that the substance recovered 

 from the urine in this experiment is closely related in its prop- 

 erties to the Bence Jones protein, for Simon and others have 

 shown that the Bence Jones protein is rendered insoluble in 

 water after it has been precipitated by alcohol and allowed 

 to stand in the reagent. 



I may also remark here, in regard to the reaction produced 

 by potassio-mercuric iodide in the non-thymolized urine 

 from this dog (page 23), that, since the animal was a male, it 

 was thought that perhaps the reaction was due to the presence 

 of spermatic fluid, a point to which Bence Jones drew atten- 

 tion in his original paper. He says "a similar body to Bence 

 Jones protein may be detected in pus and in the secretion 

 from the seminal vesicles." This reaction was obtained, 

 however, in the urines from 3 bitches and 10 other dogs, even 

 after catheterization. 



Third Experiment. At 11.30 A.M., December 24, 1908, 

 0.7801 gram of substance (A, 2, d) dissolved in 8 cc. of 0.85 

 per cent. NaCl was injected subcutaneously into a bitch. 

 The weight of the animal was 7.1 kilos. The amount of sub- 

 stance per kilo was 0.1099 gram. The dog showed no ab- 

 normal symptoms. 



The urines were subjected to the usual treatment. The 

 solution of the (NH 4 ) 2 SO 4 precipitate from fraction No. i 

 was dialyzed until free from sulphate. At this point a small 

 amount of precipitate was obtained when the solution was 

 added to alcohol. 



Certain urinary data are given in Table VI (page 29) . 



