30 



C. SUBCUTANEOUS INJECTION OF PRODUCTS OF THE TRYPTIC 

 DIGESTION OF OssEOAiyBUMOiD. Only one experiment was 

 conducted in this particular connection. The urine of the dog 

 before injection gave no precipitate on heating nor on the 

 addition of picric acid or trichloracetic acid. 



The urine was saturated with (NH 4 ) 2 SO 4 at the boiling tem- 

 perature, and a dark granular precipitate obtained, which 

 was slightly soluble in water yielding a yellow colored solution. 

 This yellow solution gave negative results in all the protein 

 color tests except the biuret, thus proving the presence of 

 urobilin; nor did the solution give a protein reaction with 

 picric acid or trichloracetic acid. 



The insoluble matter gave no protein color tests and was 

 soluble in acid, depositing crystals of uric acid from an acid 

 solution on evaporation. 



0.9145 gram of the product obtained from the digestion 

 filtrate by saturation with (NH 4 ) 2 SO 4 (A, i, b) was dissolved 

 in ii cc. of 0.85 per cent. NaCl. This was injected subcutane- 

 ously into a dog at 10.45 A.M., March 5, 1909. 



The weight of the dog was 7 kilos. The amount of the sub- 

 stance injected per kilo of the dog's weight was 0.1306 gram. 

 No unfavorable effects were noted. 



Certain urinary data are given in Table VII (page 31). 



The fractions of urine from this dog, which are referred to in 

 Table VII, were saturated with (NH 4 ) 2 SO 4 at the boiling 

 temperature. All the fractions, except No. i, yielded a con- 

 siderable amount of proteose-like precipitate, especially frac- 

 tion No. 3. The precipitates produced in the fractions were 

 dissolved in water and tested with the results shown in Table 

 VIII (page 32). 



The small amounts of (NH 4 ) 2 SO 4 precipitates obtained 

 from the above fractions that were insoluble in water did 

 not give any protein reactions, and were almost completely 

 soluble in HC1, from which solutions crystals of uric acid were 

 obtained on evaporation. 



