34 



place before the addition of the excreted substance. The 

 precipitates dissolved on further heating and became more 

 marked on cooling. 



Summary of Results of Procedures B-C. i . After the subcu- 

 taneous injection of products of peptic digestion of osseoalbumoid 

 the urine in no instance gave the heat-precipitation test, but 

 the urine gave the proteose test on addition of picric acid 

 or trichloracetic acid. An aqueous solution of the (NH 4 ) 2 SO 4 - 

 saturation precipitate gave the heat-precipitation test and 

 the proteose test with picric acid and trichloracetic acid. 



2. The substance excreted in the urine became insoluble 

 after its precipitation with alcohol, thereby resembling the 

 primary proteins rather than the proteoses. 



3. Thymolized urines give a reaction with potassio-mer- 

 curic iodide that resembles that given by the proteoses with 

 the same reagent. 



4. The urine from normal dogs also gave a reaction with potas- 

 siomercuric iodide that resembles the proteose reaction with 

 this reagent. The relation between this reaction and the 

 presence of kynurenic acid and "diamines," in dog's urine, 

 is being studied. 



5. After the subcutaneous injection of products obtained 

 by tryptic digestion of osseoalbumoid, no heat- precipitation 

 test was obtained in the urine, but the urine gave a good 

 proteose test with picric acid and trichloracetic acid. An 

 aqueous solution of the (NH 4 ) 2 SO 4 -saturation precipitate gave 

 a marked heat-precipitation test and also proteose tests with 

 picric acid and trichloracetic acid. 



6. When some of the substance excreted in the urine was 

 isolated and its aqueous solution concentrated to a syrupy 

 consistency and added to urine of 1.020 specific gravity in 

 varying proportions, no heat-precipitation test was obtained. 

 The urine, however, gave marked tests for proteose with picric 

 acid and trichloracetic acid. 



7. The injection of these products did not produce any 

 untoward effects. 



8. It seems fair to assume, on account of the fact that the 

 heat-precipitation test was not obtained in the urine direct 



