36 



mixture placed in an incubator at 39 C. At the end of a 

 week, about one-half of the material was digested. 1 



After twelve days' digestion of the osseoalbumoid by the 

 leucocytic material, it was observed that the amount of un- 

 digested matter did not diminish on the two succeeding days. 

 The mixture was then filtered, and the filtrate neutralized 

 and saturated with (NH 4 ) 2 SO 4 at the boiling temperature. 

 A very large amount of proteose-like material was obtained. 

 Even after repeated treatment of this precipitate with water 

 a considerable amount of substance remained insoluble. This 

 insoluble portion gave all the protein color reactions, but no 

 proteose tests, and proved to be an alkali albuminate. That 

 portion of the precipitate which was soluble in water was 

 dialyzed free from sulphate and concentrated speedily by 

 evaporation at 40 C. On heating an aqueous solution of 

 the above precipitate a marked cloudiness was produced, 

 which was not changed on further heating, nor by the addi- 

 tion of acid. 



With picric acid, trichloracetic acid, or potassio-mercuric 

 iodide, the solution gave very abundant precipitates which 

 dissolved almost completely on further heating and were 

 more marked on cooling. On addition of concentrated HNO 3 

 a precipitate was produced which dissolved completely on 

 heating and returned on cooling. 



The extract of leucocytes did not give a precipitate on heat- 

 ing, nor when allowed to undergo autolysis under toluol. 

 On saturating this autolyzed extract with (NH 4 ) 2 SO 4 a small 

 amount of precipitate came down, which, after solution in 

 water, did not respond to the heat-precipitation test. 



The osseoalbumoid that was not digested after two weeks' 

 treatment with the dried leucocytic product was transferred to 



1 The following references to enzymes from leucocytes may be noted : 

 Longcope and Donhauser: Jr. Exp. Med., 1908, 10, p. 618. Opie: 

 Jr. Exp. Med., 1905, 7, pp. 316, 759; 1906, 8, pp. 536,410. Opie and 

 Barker: 1907, 9, p. 207. Jochman: Zt. f. Hyg. u. Inf. Krank., 1908, 61, 

 p. 71. Jochman and Lockemann: Beitrage. f. ch. Phys. u. Path., 

 1902, 2, p. 449. Jochmann and Miiller: Munch. Med. Woch., 1906, 53, p. 

 1392, 1507, and 2002; also, Verhand. d. Kong. f. inn. Med., Wiesbaden, 

 1907. 



