38 



K. SUBCUTANEOUS INJECTION OF PRODUCTS OBTAINED IN 

 THE LEUCOCYTIC PROTEOLYSIS OF OSSEOALBUMOID. 2.3060 

 grams of the proteose-like product (D) were dissolved in 1 1 cc. 

 of 0.85 per cent. NaCl. This solution was injected subcu- 

 taneously into a bitch, the subject of a former experiment. 

 The weight of the dog was 7 kilos. The amount of the sub- 

 stance injected per kilo of dog's weight was 0.33 gram. In- 

 jection occurred at n A.M., March 16, '09. No untoward 

 effects were noticed. 



Certain urinary data are given in Table IX (page 39). 



The several fractions of urine from this dog, which are re- 

 ferred to in Table IX, were saturated with (NH 4 ) 2 SO 4 at the 

 boiling temperature. Fractions 2, 4 and 5 gave fair amounts of 

 precipitates. These precipitates were found to be very slightly, 

 if at all, soluble in water. They were insoluble in NaCl, 0.2% 

 HC1 and C 2 H 5 OH, but soluble in 0.5% N^COg. The insoluble 

 material gave very marked responses to the Millon and 

 xanthoproteic tests. The filtrate from the material insoluble 

 in water gave no reactions with picric acid, Millon's reagent 

 or potassio-mercuric iodide. 



It was thought that this excreted substance might be nu- 

 cleoalbumin. Some of the original urine was therefore ren- 

 dered amphoteric to litmus, heated to boiling and, while 

 briskly boiling, treated with acetic acid drop by drop. A 

 white, flaky precipitate was produced, which was filtered off, 

 and which gave the following reactions: 



(1) Soluble in excess of 0.2 per cent. HC1. 



(2) On being boiled with 2 per cent. HC1 until a dark color 

 formed, neutralized and tested for reduction with Fehling 

 solution, no reduction was obtained. 



(3) Gave positive test for phosphates on evaporation, 

 fusion with Na^Og and NaNO 3 , and treatment with dilute 

 HNO 3 and ammonium molybdate solution. The original 

 precipitate was washed till the washings gave no test for 

 phosphates before the fusion was made. 



These results show that the substance excreted was prob- 

 ably nucleoalbumin. No substance giving these reactions of 

 nucleoalbumin was obtained from the urine before the in- 



