4 o 



jection of the leucocytic proteolytic product of osseoalbumoid. 

 Evidently the injected product was either modified in some 

 way or nucleoalbumin present in it was excreted as such while 

 the rest of the material was retained. 



F. INTRAVENOUS INJECTION OP PRODUCTS OBTAINED IN 

 THE LEUCOCYTIC PROTEOLYSIS OF OSSEOALBUMOID. i. First 

 Experiment. 0.865 gram of the proteose-like product (D) was 

 dissolved in 30 cc. of 0.85 per cent. NaCl solution. This was 

 injected intravenously into a dog, the subject of a previous 

 experiment, at the rate of i cc. a minute, March 23, 1909. 

 The animal was under the influence of ether during the in- 

 jection procedure. No untoward symptoms developed. 



Certain urinary data are given in Table X (page 41). 



The fractions of urine from this dog, which are referred to 

 in Table X, were saturated with (NPI 4 ) 2 SO 4 at the boiling 

 temperatures and the precipitates dissolved in water and 

 tested with the results indicated in Table XI (page 42). 



The results of this experiment show that when the sub- 

 stance reached the blood stream it was excreted unchanged 

 through the kidneys, and when present in sufficient quan- 

 tities in the urine it gave a fair heat-precipitation test and 

 marked reactions with picric and trichloracetic acids. 



Of course it must be remembered that the amount of sub- 

 stance that was present in the fractions of urine was quite 

 small in comparison with the amounts excreted in myelo- 

 pathic proteosuria. Possibly, in the presence of larger amounts 

 of the substance, the heat reaction would be more marked. 



The subject of this experiment was accidentally killed a week 

 later during the ether anesthesia while preparation was 

 being made for another intravenous injection. At autopsy 

 a hemorrhagic peritonitis was found with about 50 cc. of 

 blood in the peritoneal cavity. 



2. Second Experiment. 0.4 gram of the proteose-like product 

 (D) was dissolved in a small volume of physiological salt 

 solution and injected intravenously into a dog at 2 P.M., 

 March 30, 1909. 



Certain urinary data are given in Table XII (page 43). 



