44 



The fractions of urine from this dog, which are referred to 

 in Table XII (page 43), were saturated with (NH 4 ) 2 SO 4 at the 

 boiling temperatures, and the precipitates dissolved in water 

 and tested with the results indicated in Table XIII (page 43). 



The result of this experiment confirms that of the previous 

 one. The injected substance was excreted in the urine, giving 

 a fair heat-precipitation test, and marked picric and trichlor- 

 acetic acid tests, both in the urine itself and in the solution 

 of the (NH 4 ) 2 SO 4 precipitate from the urine. The substance ex- 

 creted in the urine was shown not to have been nucleoalbumin. 

 It was like proteose in its responses to the tests employed. 



3. Summary of Results of Procedures E-F. a. After the 

 subcutaneous injection of an aqueous solution of the product 

 obtained by the leucocytic proteolysis of osseoalbumoid (D), 

 the urine did not give the heat-precipitation test, but did 

 give proteose tests with picric acid and trichloracetic acid. 



b. The substance excreted in the above case was probably 

 nucleoalbumin either present in the injected material to begin 

 with and excreted unchanged, or formed from the substance 

 injected. 



c. After intravenous injections of an aqueous solution 

 of the product obtained by the leucocytic proteolysis of osseo- 

 albumoid the urine gave a fair heat-precipitation test and 

 marked proteose tests with picric acid and trichloracetic 

 acid. 



d. The substance excreted after the intravenous injections 

 was apparently proteose and not nucleoalbumin. y 



e. In no case did the injected substance produce untoward 

 symptoms. 



/. The results of the heat-precipitation test following 

 intravenous injections suggest that possibly the Bence Jones 

 protein is formed by the action of enzymes of the bone-marrow 

 upon osseoalbumoid and that this product produced by their 

 action is eliminated through the kidneys after it reaches the 

 blood stream. 



G. INTRAPERITONEAL INJECTION OF OSSEOALBUMOID. One 

 gram of osseoalbumoid, prepared by Hawk and Gies for ele- 



