45 



mentary analysis, was suspended in water. The mixture 

 was heated to boiling, cooled and injected intraperitoneally 

 into a large dog at 3 P.M., March 25, 1909. 



Certain urinary data are given in Table XIV (page 46). 



It was found that the substance excreted consisted of serum 

 proteins. No proteose-like material could be detected. 



The urine before injection gave no reaction with picric acid 

 or trichloracetic acid. With potassio- mercuric iodide a 

 slight precipitate was obtained that did not change on further 

 heating. On saturation with (NH 4 ) 2 SO 4 a small amount 

 of a dark granular precipitate was obtained that consisted of 

 urates, urobilin, and a small amount of an insoluble residue 

 which gave no reaction for protein. 



On March 3oth, the dog died and, on autopsy, a very intense 

 hemorrhagic peritonitis was found with over a liter of bloody 

 fluid in the peritoneum. No proteose could be detected in 

 this fluid. It was hoped that in the experiment just described, 

 the osseoalbumoid would cause an inflammatory reaction and 

 then undergo a proteolysis occasioned by the enzymes of the 

 leucocytes in the exudate and that the product of this digestive 

 action would be taken up by the blood and undergo excretion. 

 Such conditions would bring about a more natural leucocytic 

 proteolysis of osseoalbumoid and would effect a process more 

 nearly comparable to that supposedly taking place in multiple 

 myeloma. On account of the danger of the intraperitoneal 

 injection, it was decided to attempt to induce more favorable 

 conditions by making an intrapleural injection of osseoal- 

 bumoid as described below. 



H. INTRAPLEURAL INJECTION OF OSSEOALBUMOID. 0.4 

 gram of osseoalbumoid (from the same product as that used 

 for the intraperitoneal injection G) was suspended in 10 cc. 

 of physiological salt solution, heated to boiling, cooled, and 

 injected jnto the left pleural cavity of a dog, at 4 P.M., April 



3, 1909- 



The urinary tests were negative (Table XV (page 46)). 



All the fractions of urine from this dog (Table XV) were 

 saturated with (NH 4 ) 2 SO 4 but fraction 4 (April 7) was the 

 only one that gave a proteose-like precipitate. A solution 



