47 



of this precipitate gave a positive reaction for proteose 

 when tested with potassio-mercuric iodide, picric acid and tri- 

 chloracetic acid. On heating an aqueous solution of the 

 precipitate produced by (NH 4 ) 2 SO 4 , a marked turbidity en- 

 sued that did not disappear completely after continued heat- 

 ing, and was more marked on cooling. 



On April lyth, two weeks after the intrapleural injection 

 the dog was killed. At autopsy the left lung and pleura 

 were found to be normal, with no evidence of any osseoalbu- 

 moid present. 



The results of the tests on the urine in this experiment 

 indicate that the injected osseoalbumoid was taken up by 

 the blood and the substance excreted. In one fraction of 

 the urine, obtained four days after the injection, a substance 

 was present which was precipitated by picric acid, the product 

 thus thrown down dissolving on warming and reappearing 

 on cooling apparently proteose. 



Summary of Results of Procedures G-H. i. Intraperitoneal 

 injection of osseoalbumoid, as a method for the production of 

 the autolytic derivatives of osseoalbumoid in the exudate 

 resulting from its injection, is apparently too unsatisfactory 

 a process for use in such experiments. 



2. The urine four days after the intrapleural injection of 

 osseoalbumoid yielded an (NH 4 ) 2 SO 4 -saturation precipitate 

 whose aqueous solution gave a pronounced heat-precipitation 

 test and marked proteose reactions with picric acid and tri- 

 chloracetic acid. 



VII. SUMMARY OF GENERAL CONCLUSIONS. 



1. The peptic digestion of osseoalbumoid yielded a water- 

 soluble substance precipitable by saturation with (NH 4 ) 2 SO 4 . 

 In one instance this material gave a heat-precipitation test, 

 but the reaction was not as marked in its sharpness as the same 

 test when applied to elastose. Negative results were obtained 

 when the test was applied to two other similar products ob- 

 tained in the peptic digestion of osseoalbumoid. 



2. After the subcutaneous injection of the material produced 

 by the peptic digestion of osseoalbumoid, the urine did not 



