4 8 



yield a heat-precipitation test, but gave a positive reaction 

 with picric and trichloracetic acids. The (NH 4 ) 2 SO 4 pre- 

 cipitate from the urine in this experiment gave positive heat 

 as well as picric acid and trichloracetic acid tests. 



3. The heat-precipitation test in the urine is determined 

 by the amount of substance excreted as well as by the reac- 

 tion of the urine and the quantity of salts present. 



4. After the subcutaneous injection of the material pro- 

 duced by the peptic digestion of osseoalbumoid, an alcoholic 

 precipitate of the substance excreted in the urine is rendered 

 insoluble on standing under alcohol, thereby showing one of 

 the characteristics of the Bence Jones protein, and departing 

 from the usual properties of proteoses. 



5. After the subcutaneous injection of the substance obtained 

 by the tryptic digestion of osseoalbumoid, it was excreted by 

 the urine but it did not give a heat precipitation test. 



6. Osseoalbumoid undergoes proteolysis when subjected 

 to the action of the enzyme of leucocytes that acts in an al- 

 kaline media. A solution of the (NH 4 ) 2 SO 4 precipitate of this 

 digestive mixture gave a heat precipitation test as well as 

 reactions with picric acid and trichloracetic acid. 



7. The material produced by the leucocytic proteolysis of 

 osseoalbumoid, after its subcutaneous injection, was found to 

 have been modified in the body and was excreted, in whole 

 or in part, as a nucleoalbumin-like substance in the urine. 



8. Intraperitoneal injection could not be used as a satisfac- 

 tory method of favoring autolysis of osseoalbumoid on account 

 of the peritonitis produced. 



9. Osseoalbumoid was absorbed, after its intrapleural 

 injection, and a digestive product of it was excreted in the 

 urine. This product in the urine did not give a heat-precipita- 

 tion test, but a solution of the (NH 4 ) 2 SO 4 precipitate from 

 the urine gave this reaction as well as one with picric acid 

 and trichloracetic acid. 



10. Some of the results indicate that the Bence Jones protein 

 may be formed from osseoalbumoid by the action of enzymes 

 present in the bone marrow. 



