8 



and described by Kiihne in 1869. It has since been the subject 

 of many investigations, especially by Matthes, Bllinger, 

 Magnus-Levy, Jochman and Schumm, Bradshaw, Park, 

 Moffat, Simon, and others. 



Ellinger succeeded in obtaining the Bence Jones protein 

 in small amounts from diseased bone marrow and ascitic 

 fluid. Virchow found it in the bone marrow in cases of os- 

 teomalacia, so called. Barr could not find in the bone marrow 

 or bone tumor substance, any trace of the Bence Jones protein 

 or of enzymes. Wood claims to have separated the Bence 

 Jones protein from a portion of bone affected by multiple myel- 

 oma, but could not obtain it from the bone marrow in any 

 other portion of the body of the patient. Askanazy was able 

 to demonstrate its presence in the bone marrow of a case of 

 multiple myeloma but was unable to find it in the blood from 

 this patient. Lowy could not detect a trace of the Bence 

 Jones protein in the marrow of the affected ribs and humerus 

 of Kalischer's case. Weber, however, was able to prove the 

 presence of a substance giving reactions similar to those 

 of the Bence Jones protein, in the vertebrae and ends of the 

 femur in a case of multiple myeloma, but he could not detect 

 this substance in any organ or tissue. Bruce, Lund, and 

 Whitcomb found, in a case of multiple myeloma, that the 

 fluid obtained from an affected bone, after sawing through 

 it, gave the reactions of the Bence Jones protein. Ribbinik 

 could not find the Bence Jones protein in the bone marrow 

 substance of the case studied by him. Fleischer, however, has 

 found a substance giving the reactions of the Bence Jones 

 protein in normal bone marrow. 



Bradshaw and Warrington, in an analysis of a rib affected 

 with multiple myeloma, found the relation of organic and in- 

 organic substances to be practically normal. Magnus-Levy 

 and also Grutternick and deGraaf have succeeded in obtaining 

 the Bence Jones protein in crystalline form. 



Moitessier on subjecting the Bence Jones protein to gastric 

 digestion obtained acidalbumin, primary proteoses (except 

 heteroproteose), secondary proteoses and peptone. After 

 peptic digestion of the Bence Jones protein, Simon could not 



