66 



PRACTICAL PHYSIOLOGY. 



[VII. 



(b.) Spread a sheet of white paper on a table in a good light opposite a 

 window, and on it place two ha-matinometers side by side (fig. 31, D). See 

 that they arc water-tight. If not, anoint the edges of the glass plates with 

 vaseline to make them water-tight. 



(c.) Take 10 cc. of the standard solution of huemoglobin and dilute it with 

 50 cc. of water, and j)lace it in one of the haematinometers. 



{d.) Weigh 5 grams of the blood to be investigated, and dilute it with 

 water exactly to 100 cc. 



{e.) Place 10 cc. of this desper tinted blood {d.) into the second haematino- 

 meter. 



(/. ) Fill an accurately graduated burette with distilled water, place it over 

 the second ha^matinometer {e. ), and dilute the blood in it until it has precisely 

 the same tint as the standard solution in the other hfematinometer. Note the 

 amount of water added. The two solutions must now contain the same 

 amount ofhaenjoglobin. 



'EtXB.m.T^le {HuppeSeyler). — 20.186 grams of defibrinated blood were diluted 

 with water to 4CX) cc. To the 10 cc. of this placed in a haematinometer, 38 



Fig, 39.— Zeiss'.s Microspcctroscope after Al be. 



Fig. 40 —Adjustable Slit in Fig. 39, A. 



cc. of water had to be added to obtain the same tint as that of the standard 

 solution, so that the volume of water which would require to be added to 

 dilute the whole 400 cc. would !»e 1520 cc, thus— 



10 : 400 : : 38 : a; 

 X = 1520 cc. 



By adding 1520 cc. of distilled water to the 400 cc. of blood solution, we get 

 1920 cc. of the same tint or degree of dilution as the standard solution. 



The standard solution on analysis was found to contain 0.145 gi'ams of 

 hemoglobin in 100 cc, so that the total amount of haemoglobin in the diluted 

 Mood is found, thus — 



100 : 1920 : : o. 145 : x 

 X = 2.784 grams. 



