i8 



in the nutrient solution. It is less tiresome and more certain of results to carry out the infection 

 with smut spores instead of conidia, if they have been prepared beforehand for direct germination 

 in the way stated above. This use of smut spores for infection becomes indeed a necessity if 

 no conidia occur in spore germination when one can depend only on the use of spores, as may 

 occur in smut spores of barley and wheat. 



The same spore material used in spraying germinating seedlings may now be used in still 

 a second case, in order to infect sufficiently the best compost earth. This earth was mixed in 

 the third case with horse manure. The spores were abundantly blown on to it with the atomizer 

 and mixed in and the earth thus infected was used for covering the sown grain. In the fourth 

 case, independent of these three cases, the grain in a dry condition was mixed with dry smut 

 spores and then sown. 



