Jan. 1917 Poisonous Properties of Milk Vetch. 63 



Mixture digested 24 hours at a temperature of 37.5 C., 

 concentrated to small bulk and diluted with 2 oz. of water. 

 No. 2. 



Leaves (dry) 3 oz. 



Alcohol (95% ) 10 oz. 



Treated the same as No. i. 

 No. 3. 



Seeds and pods (dry) 3 oz. 



Water 15 oz. 



Digested for 24 hours at 37.5 C. Aqueous portion sep- 

 arated and concentrated to 2 oz. 

 No. 4. 



Leaves and stems (dry) 46 oz. 



Water 350 oz. 



Same treatment as No. 3, concentrated to 75 oz. 

 No. 5. 



Stems (dry) 6 oz. 



\Yater 12 oz. 



Treated as in No. 3, concentrated to 5 oz. 

 No. 6. 



Leaves and stems (dry) 87 oz. 



Water 150 oz. 



Same treatment as in No. 3, concentrated to 25 oz. 

 No. 7. 



Whole plant (dry) 22 oz. 



Water 1 50 oz. 



Digested 48 hours at 37.5 C. Aqueous portion sep- 

 arated and concentrated to 25 oz. Treated with slight ex- 

 cess of 'basic lead acetate. Lead precipitate removed, 

 washed, and suspended in large volume of water. Lead 

 precipitated as lead sulphide. Remaining solution con- 

 centrated to 5 oz. Labeled Solution "A". 



That portion of the original water extract not precip- 

 itated by basic lead acetate was treated with hydrogen 

 sulphide to remove lead and concentrated to a volume of 

 5 oz. Labeled Solution "B". 



