58 Bulletin No. 207. 



period of 9 days elapsed. In some cases death occurred with- 

 out noticeable premonitory symptoms, while other infected 

 animals died in 24 hours after the first symptoms were ob- 

 served or remained decumbent and moribund for 3 or 4 days 

 until death. An individual tolerance as well as the amount 

 of toxin administered were possible factors in the variations 

 observed. The gross lesions in infected horses varied in in- 

 tensity. In animals subacutely affected a gross anatomic pic- 

 ture of the lesions observed in natural outbreaks of forage 

 poisoning was frequently presented. 



Cattle: Cattle seemed more resistant to B. botulinus than 

 horses and mules. A Jersey calf weighing 250 pounds was 

 fed at 2 day intervals for 4 feedings 5 to 8 cc. of unfiltered 

 broth culture of B. botulinus. The toxin was thoroly mixed 

 with bran and fed immediately. No temperature reaction or 

 external manifestations of discomfort were observed in this 

 animal. A mature Jersey cow weighing 900 pounds received 

 0.1 cc. B. botulinus in broth subcutaneously on November 14th, 

 1916. On November 20th, 4 cc. and on November 27th, 20 cc. 

 were given in a similar manner. There was no thermal reac- 

 tion or clinical change noted in this animal following the in- 

 jections, other than a mild transitory edema at point of in- 

 oculation. 



Sheep: On October 7th, 1916, a mature buck sheep (No. 1) 

 was fed cornmeal in which 7 cc. of B. botulinus in broth 

 culture were thoroly mixed. On the three succeeding days 

 this animal was fed daily 7 cc. of broth culture mixed with 

 wholesome feed. On October llth and 12th, 7 cc. were fed; 

 on October 13th, 14 cc. ; on October 14th, 7 cc. ; on October 

 16th, 10 cc. On November 2nd, 7 cc. of broth culture were 

 administered subcutaneously ; on November 14th, 0.1 cc. ; on 

 November 27th, 10 cc., followed on December 4th by 10 cc. 

 The amount was increased on December 12th to 15 cc., and on 

 December 16th, to 70 cc. The feeding and injections of B. 

 botulinus as enumerated produced no manifest change other 

 than edema at points of inoculation. 



A pregnant ewe (No. 2) was given 300 cc. of broth cul- 

 ture of B. botulinus mixed in wholesome feed. Clinical mani- 



