Studies in 'For age Poisoning V. 59 



festations of botulism, i. e., salivation, marked weakness and 

 intermittent decumbency, were observed on the seventh day 

 (Fig. 5). Death occurred on the tenth day. The gross lesions 

 were similar to those in horses fatally afflicted as the result 

 of ingesting many times smaller amounts of the toxin. 



Goats: A mature goat (No. 1) weighing 95 pounds was 

 fed and injected subcutaneously with B. botulinus at intervals, 

 receiving amounts approximately equivalent to those given 

 sheep No. 1. On one occasion, following the subcutaneous in- 

 jection of a broth culture of B. botulinus, diarrhea was ob- 

 served. Recovery without treatment soon followed and the 

 goat remained apparently healthy. 



A female goat (No. 2) suckling a kid was given 300 cc. 

 broth culture mixed in wholesome feed and, like the pregnant 

 ewe, manifested symptoms of botulism in 7 days (Fig. 6), 

 followed by death after several days of decumbency. 



Swine : A thrifty cholera immune shoat, weighing 75 

 pounds, was fed daily from October 7th to October 16th, 1916, 

 7 cc. of broth culture of B. botulinus mixed in cornmeal. No 

 ill effect was noted during this time. After October 16th 

 wholesome feed was supplied and the animal remained appar- 

 ently normal. Subcutaneous injections of 5 cc. unfiltered 

 broth cultures of B. botulinus proved fatal in two cholera im- 

 mune shoats, weighing 75 pounds each. Difficult respiration, 

 loss of appetite, salivation, extreme weakness, muscular 

 tremors and decumbency were observed before death. On 

 autopsy engorgement of the liver, ecchymoses on the heart, 

 areas of congestion on the inner wall of the large and small 

 intestines, edematous and congested visceral lymph glands and 

 injection of the mesentery were observed. 



Guinea Pigs : Guinea pigs proved very susceptible to B. 

 botulinus administered subcutaneously and per os. 0.05 cc. 

 broth culture by the mouth proved fatal in some instances in 

 24 hours. Smaller amounts of B. botulinus administered sub- 

 cutaneously proved fatal in 36 to 96 hours. Pregnancy seemed 

 to render these animals more susceptible. Equal amounts of 

 toxin proved more rapidly fatal in pregnant than in non- 

 pregnant guinea pigs of equal weight. 



