64 Bulletin No. 207. 



forage extracts mentioned, and in the alfalfa and corn silage 

 extracts sown with Fusarmm sp. and B. botulinus there was 

 gas production. After 12 days 0.2 cc. from each of the inocu- 

 lated extracts was administered by the mouth to guinea pigs. 

 A pig receiving by the mouth 0.2 cc. of the alfalfa extract of 

 Fusarium sp. and B. botulinus in association died in 45 hours, 

 showing before death extreme weakness and salivation. 0.2 

 cc. of the corn silage extract culture of Fusarium sp. and B. 

 botulinus, by the mouth, sufficed to cause death in a guinea 

 pig weighing 400 gms. in 40 hours. A pig receiving by the 

 mouth 0.2 cc. of the oat extract culture of Fusarium sp. and 

 B. botulinus in association died in 4 days. There was no evi- 

 dence of disease in the pig receiving 0.2 cc. of the culture of 

 Fusarium sp. associated with B. botulinus in corn extract, nor 

 in any of the pigs receiving cultures of Fusarium sp. alone. 

 The symptoms and anatomic alterations observed upon autopsy 

 as the result of artificial infection of B. botulinus and Fu- 

 sarium sp. in association in the alfalfa, corn silage and oat 

 extracts were similar to those seen in pigs fatally infected with 

 broth cultures of B. botulinus. 



Growth did not develop in extracts of alfalfa, oats, corn 

 and corn silage, made neutral to litmus, after being sown 

 with B. botulinus and incubated anaerobically. Guinea pigs 

 receiving 0.5 cc. by the mouth remained healthy. Tubes of 

 sterile oat decoction with a reaction to phenolphthalein of 

 +0.8 were inoculated with Fusarium sp. and B. botulinus simul- 

 taneously and incubated at room temperature under aerobiosis 

 for 12 days. The culture, in a dose of 0.2 cc., by the mouth, 

 sufficed to cause the death, in 30 hours, of a guinea pig weigh- 

 ing 500 gms. Corn silage extract, with a reaction to phenol- 

 phthalein of +2.6 when sown with Fusarium sp. and B. botulinus 

 developed growth, with production of gas. When 11 days 

 old 0.5 cc. of this culture was administered by the mouth to 

 a guinea pig weighing 500 gms., followed by characteristic 

 symptoms and death in 6 days. Pork-peptone broth, made 

 distinctly acid to litmus, altho not furnishing a suitable 

 medium for B. botulinus alone under anaerobic conditions, 

 proved favorable for its growth in association with Fusarium 



