Studies in Forage Poisoning V. 69 



ratory index G. P. No. c-91-M) received by the mouth 0.2 cc. 

 of the anaerobic organism c-91 grown in glucose pork broth. 

 On February 15th the pig was seriously afflicted, unable to 

 stand, salivated and moribund. At 4 p. m. a photograph was 

 obtained (Fig. 10). The pig was found dead the morning of 

 February 16th. Plantings from the blood and organs of guinea 

 pig G. P. No. c-91-M remained sterile. 



Introduction of the organism c-91, in unfiltered broth 

 culture, in amounts of 0.03 to 0.05 cc., by the mouth, proved 

 fatal in 24 to 36 hours, to guinea pigs weighing from 250 to 

 600 gms. 



The pathogenic effect on guinea pigs of the organism c-91 

 isolated from the caecum of a horse that succumbed to the 

 Griffith oat water, suggested experimental trials upon horses. 

 At noon on February 14th, 1917, a healthy thorobred mare, 

 No. 94, weighing 950 pounds, was allowed to ingest 4 cc. un- 

 filtered broth culture disguised in one quart of wholesome 

 oats. At 2 o 'clock she had completely ingested the oats. This 

 animal remained apparently healthy until the morning of 

 February 19th, at which time there was observed a profuse 

 salivation, clicking sound in throat, unsteady movement, 

 stupor, walking in circle, constant shifting of the weight from 

 one foot to the other, more noticeable in the hind quarters, 

 frequent yawning and chewing, slight mucous discharge from 

 nostrils. At 11 a. m. a photograph of the animal was ob- 

 tained (Fig. 11). During the afternoon the animal was de- 

 cumbent, rising with difficulty, and on the following morning, 

 February 20th, at 7 a. in., became permanently decumbent. 

 At intervals the feet were moved violently, at other times a 

 quiet restful attitude was presented. No food or water was 

 taken after the morning of the 20th, and death occurred early 

 the morning of the 23rd. 



Upon autopsy the following observations were made : 

 Emaciation very marked. Abrasions on hip, shoulder, orbit, 

 tongue and fetlocks, self-inflicted in decumbency, with bruised 

 and congested areas in the corresponding subcutaneous tissue. 

 Mucous discharge about external nostrils. In the nasal mem- 

 brane scattered petechiae were observed. Lungs slightly con- 



